Application
Western blot analysis of RIP/Rab expression in non-transfected 293T: (A), mouse RIP/Rab transfected 293T: (B) and Jurkat (C) whole cell lysates.| Product Name | RIP/Rab Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | HIV-1 Rev is the prototype of a class of retroviral regulatory proteins that control the sequence-specific nuclear export and translation of a class of incompletely spliced HIV-1 mRNAs that encode viral structural proteins. In the absence of Rev, these late viral RNAs remain sequestered in the nucleus until they are either spliced or degraded. The protein designated Rev interacting protein (RIP) or Rev/Rex activation domain-binding protein (Rab) contains 562 amino acids. RIP/Rab has been identified as a cellular cofactor that binds not only to the HIV-1 Rev activation domain, but also to equivalent domains of other Rev and Rex proteins. On the basis of these findings it has been speculated that RIP/Rab is required for the Rev response and thus for HIV-1 replication. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RIP/Rab of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 263-562 mapping at the C-terminus of RIP/Rab of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 263-562 mapping at the C-terminus of RIP/Rab of human origin |
| Entrez Gene | 3267 |
|---|
Application
Western blot analysis of RIP/Rab expression in non-transfected 293T: (A), mouse RIP/Rab transfected 293T: (B) and Jurkat (C) whole cell lysates.| Application Notes | recommended for detection of RIP/Rab of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
