Application
Western blot analysis of phosphorylated Stat4 expression in untreated (A) and IL-4-treated (B) HeLa whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplamic staining.| Product Name | p-Stat4 Antibody (Tyr 693) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Membrane receptor signaling by various ligands, including interferons and growth hormones, induces activation of Jak kinases, which then leads to tyrosine phosphorylation of the Stat transcription factors. Upon activation by tyrosine phosphorylation, Stat proteins dimerize, translocate to the nucleus and bind to specific regulatory elements that control gene expression. Stat4 is most highly expressed in testis and myeloid cells and is an important element in mediating IL-12 signals. IL-12 induces sustained activation and nuclear translocation of Stat4, a process coupled to both tyrosine and serine phosphorylation of Stat4. Phosphorylation of Ser 721 of Stat4 is MEK-, ERK- and JNK-independent and p38-dependent, and is necessary for the transcriptional activity of Stat4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Tyr 693 phosphorylated Stat4 of human and rat origin and correspondingly phosphorylated Tyr 694 of mouse origin by WB, IP, IF and IHC(P) | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Tyr 693 of Stat4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Tyr 693 of Stat4 of human origin |
| Entrez Gene | 6775 |
|---|
Application
Western blot analysis of phosphorylated Stat4 expression in untreated (A) and IL-4-treated (B) HeLa whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplamic staining.| Application Notes | recommended for detection of Tyr 693 phosphorylated Stat4 of human and rat origin and correspondingly phosphorylated Tyr 694 of mouse origin by WB, IP, IF and IHC(P) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
