Application
Western blot analysis of ISCA1 expression in non-transfected: (A) and human ISCA1 transfected: (B) 293T whole cell lysates.| Product Name | ISCA1/1L Antibody (G-12) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Iron-sulfur cluster assembly (ISCA) proteins are members of the hesB/iscA family that play an essential role in many biological proceses such as the assembly of mitochondrial iron-sulfur proteins and the binding of iron-sulfur protein intermediates. ISCA1L (iron-sulfur cluster assembly 1-like) and ISCA1 (iron-sulfur cluster assembly 1), also known as ISA1, HBLD2 or hIscA, are implicated in the biogenesis of iron-sulfur clusters. ISCA1 is encoded by a gene located on human chromsome 9 whereas ISCA1L is encoded by a gene located on human chromosome 5. Chromosome 9 houses over 900 genes, comprises nearly 4% of the human genome and encompasses the largest interferon family gene cluster. Chromosome 5 contains 181 million base pairs and comprises nearly 6% of the human genome. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm or of chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ISCA1/1L of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of ISCA1/1L of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of ISCA1/1L of human origin |
| Entrez Gene | 81689 |
|---|
Application
Western blot analysis of ISCA1 expression in non-transfected: (A) and human ISCA1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of ISCA1/1L of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
