Application
Western blot analysis of Sororin expression in non-transfected: (A) and human Sororin transfected: (B) 293T whole cell lysates.| Product Name | Sororin Antibody (FL-252) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Sororin, also designated cell division cycle-associated protein 5 (CDCA5) or p35, functions as a regulator of sister chromatid cohesion during mitosis. It interacts with the APC/C complex and is found in a complex consisting of cohesion components SCC-112, MC1L1, SMC3L1, RAD21 and APRIN. The deduced human and mouse Sororin proteins consist of 252 and 264 amino acid residues, respectively, and both contain a KEN box for APC-dependent ubiquitination. Reserach demonstrates a punctated nuclear distribution of Sororin during interphase and a diffuse distribution throughout the cell during mitosis. There is no apparent concentration of Sororin on chromatin in mitotic cells and Sororin levels decrease in synchronized HeLa cells during interphase. The Sororin gene maps to chromosome 11q12.1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Sororin (CDCA5) of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-252 representing full length Sororin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-252 representing full length Sororin of human origin |
| Entrez Gene | 113130 |
|---|
Application
Western blot analysis of Sororin expression in non-transfected: (A) and human Sororin transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Sororin (CDCA5) of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
