Application
Western blot analysis of PP5 expression in non-transfected 293T: (A), mouse PP5 transfected 293T: (B) and PC-12 (C) whole cell lysates.| Product Name | PP5 Antibody (H-170) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunit have been identified, designated PP1, PP2A, PP2B and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4), is a putative member of a novel PP family. PP5, also designated Protein phosphatase T (PP-T, PPP5C), a predominantly nuclear protein which belongs to the PPP phosphatase family and the PP-T subfamily, interacts with CDC16 and CDC27. It dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1 and may be involved in mitosis and RNA biogenesis regulation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Serine/Threonine Protein Phosphatase 5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 71-240 mapping near the N-terminus of Serine/Threonine Protein Phosphatase 5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 71-240 mapping near the N-terminus of Serine/Threonine Protein Phosphatase 5 of human origin |
| Entrez Gene | 5536 |
|---|
Application
Western blot analysis of PP5 expression in non-transfected 293T: (A), mouse PP5 transfected 293T: (B) and PC-12 (C) whole cell lysates.| Application Notes | recommended for detection of Serine/Threonine Protein Phosphatase 5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
