Application
Western blot analysis of EVL expression in non-transfected 293T: (A), human EVL transfected 293T: (B) and Ramos (C) whole cell lysates.Application
Western blot analysis of EVL expression in non-transfected: (A) and human EVL transfected: (B) 293T whole cell lysates.Application
Western blot analysis of EVL expression in Ramos whole cell lysate.| Product Name | EVL Antibody (R-51) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | EVL (Ena/VASP-like protein) is an Actin-binding protein that belongs to the Mena/VASP protein family. EVL is expressed in filopodial tips and localizes to the edge of the lamellipodia and focal adhesions. In epithelial cells, EVL localizes to the membrane of the lateral domain. EVL contains an N-terminal EVH1 domain, a proline-rich core and a C-terminal EVH2 domain. Via its proline-rich domain, EVL interacts with the SH3 domain of spectrin a II and the LIM domain of TES. EVL is closely related to VASP (vasodilator-stimulated phosphoprotein) and Mena (for mammalian enabled protein). Mena is highly expressed in the developing nervous system and may be involved in growth cone motility and axon guidance; VASP is involved in the maintenance of cyto-architecture by interacting with Actin-like filaments. All three proteins, EVL, Mena and VASP, are involved in cell motility and the regulation of cytoskeletal organization and dynamics. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of EVL of human, rat and, to a lesser extent, mouse origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 326-376 mapping near the C-terminus of EVL of rat origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 326-376 mapping near the C-terminus of EVL of rat origin |
| Entrez Gene | 14026 |
|---|
Application
Western blot analysis of EVL expression in non-transfected 293T: (A), human EVL transfected 293T: (B) and Ramos (C) whole cell lysates.
Application
Western blot analysis of EVL expression in non-transfected: (A) and human EVL transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of EVL expression in Ramos whole cell lysate.| Application Notes | recommended for detection of EVL of human, rat and, to a lesser extent, mouse origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
