Application
Western blot analysis of RGS16 expression in non-transfected: (A) and mouse RGS16 transfected: (B) 293T whole cell lysates.| Product Name | RGS16 Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Regulators of G-protein signaling (RGS proteins) are a family of highly diverse, multifunctional signaling proteins that share a conserved 120 amino acid domain (RGS domain). RGS domains bind directly to activated Ga subunits and act as GTPase-activating proteins (GAPs) to attenuate and/or modulate hormone and neurotransmitter receptor-initiated signaling by both Ga-GTP and Gbg. RGS proteins shorten the lifetime of the activated G protein. RGS16 is expressed at high levels in retina. Overexpression of RGS16 inhibits G protein-coupled mitogenic signal transduction and activation of the mitogen-activated protein kinase (MAPK) signaling cascade. Rgs16 enhances the rate of GTP-hydrolysis by transducin, suggesting that Rgs16 may play a role in regulating the kinetics of signaling in the phototransduction cascade. The gene which encodes RGS16 maps to human chromosome 1q25-q31. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RGS16 of mouse, rat and human origin by WB, IP, IF and ELISA cross-reactive, to a lesser extent, with other RGS family members also reactive with additional species, including equine and canine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 103-202 mapping at the C-terminus of RGS16 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 103-202 mapping at the C-terminus of RGS16 of human origin |
| Entrez Gene | 6004 |
|---|
Application
Western blot analysis of RGS16 expression in non-transfected: (A) and mouse RGS16 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of RGS16 of mouse, rat and human origin by WB, IP, IF and ELISA cross-reactive, to a lesser extent, with other RGS family members also reactive with additional species, including equine and canine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
