Application
Western blot analysis of ASC expression in U-937 (A), SK-MEL-28 (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of ASC expression in HL-60 (A) and SK-MEL-28 (B) whole cell lysates.Application
Western blot analysis of ASC expression in non-transfected 293T: (A), mouse ASC transfected 293T: (B) and HL-60 (C) whole cell lysates.Application
Western blot analysis of ASC expression in HL-60 (A) and U-937 (B) whole cell lysates.| Product Name | ASC Antibody (H-120) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Caspase-associated recruitment domains (CARDs) mediate the interaction between adaptor proteins such as Apaf-1 and the proform of caspases (e.g., CASP9) participating in apoptosis. ASC (apoptosis-associated speck-like protein containing a CARD, also known as TMS1or PYCARD) is a member of the CARD-containing adaptor protein family. ASC is a 195 amino acid protein, containing a N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal CARD. This motif is characteristic of numerous proteins involved in apoptotic signaling. Fluorescence microscopy demonstrates a ring-like expression in some transfected cells. Immunofluorescence microscopy demonstrates that induction of apoptosis causes a CARD-dependent shift from diffuse cytoplasmic expression to punctate or spherical perinuclear aggregates. Western blot analysis shows expression of ASC in leukemia and melanoma cell lines. ASC exhibits intriguing behavior by forming an aggregate and appearing as a speck during apoptosis induced by retinoic acid and other anti-tumor drugs. The ASC gene maps to human chromosome 16p11.2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ASC of of human origin WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-120 mapping at the N-terminus of ASC of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-120 mapping at the N-terminus of ASC of human origin |
| Entrez Gene | 29108 |
|---|
Application
Western blot analysis of ASC expression in U-937 (A), SK-MEL-28 (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of ASC expression in HL-60 (A) and SK-MEL-28 (B) whole cell lysates.
Application
Western blot analysis of ASC expression in non-transfected 293T: (A), mouse ASC transfected 293T: (B) and HL-60 (C) whole cell lysates.
Application
Western blot analysis of ASC expression in HL-60 (A) and U-937 (B) whole cell lysates.| Application Notes | recommended for detection of ASC of of human origin WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
