Application
Antibodies tested include p-Rho A (Ser 188): (A,B) and Rho A (119): (C,D).Application
Western blot analysis of Rho A phosphorylation in untreated (A) and lambda protein phosphatase treated (B) mouse brain tissue extracts.| Product Name | p-Rho A Antibody (Ser 188) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1 (A and B), Rap 2, R-Ras and Ral (A and B), exhibit approximately 50% amino acid homology to Ras. The three mammalian Rho proteins (A, B and C) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function. Phosphorylation of Ser188 protects RhoA from ubiquitin/proteasome-mediated degradation in vascular smooth muscle cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ser 188 phosphorylated Rho A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 188 of Rho A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 188 of Rho A of human origin |
| Entrez Gene | 387 |
|---|
Application
Antibodies tested include p-Rho A (Ser 188): (A,B) and Rho A (119): (C,D).
Application
Western blot analysis of Rho A phosphorylation in untreated (A) and lambda protein phosphatase treated (B) mouse brain tissue extracts.| Application Notes | recommended for detection of Ser 188 phosphorylated Rho A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
