Application
Western blot analysis of PARD6B expression in RAW 264.7 whole cell lysate (A) and rat spleen (B), mouse spleen (C), mouse pancreas (D) and rat pancreas (E) tissue extracts.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.Application
Western blot analysis of PARD6B expression in HeLa (A), Caki-1 (B), MIA PaCa-2 (C), JAR (D), PC-3 (E) and Jurkat (F) whole cell lysates.| Product Name | PARD6B Antibody (M-64) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Cellular asymmetry is critical for the development of multicellular organisms. PARD (partitioning-defective) proteins play important roles in asymmetric cell division and polarized growth, whereas Cdc42 and Rac mediate establishment of cell growth and polarity and contribute to oncogenic transformation by Ras. The human PARD6, a 345 amino acid polypeptide, has a PDZ domain and a CRIB-like (Cdc42/Rac interactive binding) motif. PARD6 interacts with GTP-bound Rac and Cdc42 via this motif and with the atypical PKC isoforms PKCî/¬ and PKCΩ via N-terminal head to head association. These interactions allow formation of a ternary complex in vitro and in vivo, which is implicated in the formation of normal tight junctions at epithelial cell-cell contacts and is also involved in the polarization of mother cells before asymmetric cell division in C. elegans. PARD6 acts through PARD3 by localizing or maintaining the PARD3 protein at the cell periphery. PARD6A, also designated PAR-6å, PAR6C, TAX40 and TIP-40, is expressed in pancreas, skeletal muscle, brain and heart, and is weakly expressed in kidney and placenta. PAR6B is expressed in pancreas and in both adult and fetal kidney, and is weakly expressed in placenta and lung. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PARD6B of mouse, rat and, to a lesser extent, human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 308-371 mapping at the C-terminus of PARD6B of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 308-371 mapping at the C-terminus of PARD6B of mouse origin |
| Entrez Gene | 84612 |
|---|
Application
Western blot analysis of PARD6B expression in RAW 264.7 whole cell lysate (A) and rat spleen (B), mouse spleen (C), mouse pancreas (D) and rat pancreas (E) tissue extracts.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.
Application
Western blot analysis of PARD6B expression in HeLa (A), Caki-1 (B), MIA PaCa-2 (C), JAR (D), PC-3 (E) and Jurkat (F) whole cell lysates.| Application Notes | recommended for detection of PARD6B of mouse, rat and, to a lesser extent, human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
