Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing cytoplasmic staining of cells in germinal and non-germinal centers.Application
Western blot analysis of CTR1 expression in non-transfected 293T: (A), human CTR1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of CTR1 expression in non-transfected 293T: (A), mouse CTR1 transfected 293T: (B) and Hela (C) whole cell lysates.Application
Western blot analysis of CTR1 expression in non-transfected 293T: (A), mouse CTR1 transfected 293T: (B) and Hela (C) whole cell lysates.| Product Name | CTR1 Antibody (FL-190) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The activity of a diverse subset of enzymes relies on the essential nutrient copper (1-3). Copper uptake requires tight regulation to ensure that sufficient copper is present in the cell to drive vital cellular processes, while avoiding the accumulation of copper to toxic levels (1-3). In Saccharomyces cerevisiae, copper regulation involves several proteins. Fre1, a surface reductase, reduces and mobilizes copper outside the cell, while the Ctr1 and Ctr3 proteins function as copper transport proteins within the plasma membrane (2-4). Regulation of these proteins occurs at the transcriptional level (2-4). Under copper-deficient conditions, Mac1 binds to copper response elements (CuREs) within promoters, which contain the consensus sequence GCTC, to activate the transcription of Ctr1, Ctr3, and Fre1 (2-6). Mac1 also mediates Ctr1 degradation (2). In human, Ctr1 also mediates the uptake of cisplatin, a chemotherapeutic drug, and may modulate the sensitivity and toxicity of this drug (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CTR1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-190 representing full length CTR1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-190 representing full length CTR1 of human origin |
| Entrez Gene | 1317 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing cytoplasmic staining of cells in germinal and non-germinal centers.
Application
Western blot analysis of CTR1 expression in non-transfected 293T: (A), human CTR1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of CTR1 expression in non-transfected 293T: (A), mouse CTR1 transfected 293T: (B) and Hela (C) whole cell lysates.
Application
Western blot analysis of CTR1 expression in non-transfected 293T: (A), mouse CTR1 transfected 293T: (B) and Hela (C) whole cell lysates.| Application Notes | recommended for detection of CTR1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
