Application
Western blot analysis of JMJD2B expression in non-transfected: (A) and mouse JMJD2B transfected: (B) 293T whole cell lysates.| Product Name | JMJD2B Antibody (M-66) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | JMJD2B (JmjC domain-containing histone demethylation protein 3A) is a 1,064 amino acid protein encoded by the human gene JMJD2B. JMJD2B belongs to the JMJD2B histone demethylase family and contains one JmjC domain, one JmjN domain, two PHD-type zinc fingers and two Tudor domains. The two Tudor domains recognize and bind methylated histones and have an interdigitated structure; the unusual fold is required for its ability to bind methylated histone tails. JMJD2B is a histone demethylase that specifically demethylates Lys 9 residues of Histone H3, thereby playing a role in histone code. It does not demethylate Histone H3 Lys 4, H3 Lys 27, H3 Lys 36 or H4 Lys 20, however, and is only able to demethylate trimethylated H3 Lys-9 and has weaker activity than JMJD2A, JMJD2C and JMJD2D. JMJD2B demethyl-ation of Lysine residues will generate formaldehyde and succinate. JMJD2B is a ubiquitously expressed nuclear protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of JMJD2B of mouse and rat origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1021-1086 mapping at the C-terminus of JMJD2B of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1021-1086 mapping at the C-terminus of JMJD2B of mouse origin |
| Entrez Gene | 193796 |
|---|
Application
Western blot analysis of JMJD2B expression in non-transfected: (A) and mouse JMJD2B transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of JMJD2B of mouse and rat origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
