Application
Western blot analysis of DHS expression in non-transfected: (A) and human DHS transfected: (B) 293T whole cell lysates.Application
Western blot analysis of DHS expression in HeLa whole cell lysate.| Product Name | DHS Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Deoxyhypusine synthase (DHS) is crucial for the post-translational formation of hypusine, a modification of a specific lysine residue in eukaryotic initiation factor 5A (eIF-5A). Hypusine is formed by posttranslational modifications involving two enzymatic steps catalyzed by DHS and deoxyhypusine hydroxylase (DOHH). eIF-5A is essential for eukaryotic cell proliferation. Deoxyhypusine synthase, which belongs to the deoxyhypusine synthase family of proteins, is important for the first step in the hypusine biosynthesis pathway. It acts as a catalyst for the NAD-dependent oxidative cleavage of spermidine and the ensuing transfer of the butylamine moiety of spermidine to the eIF-5A protein, to create the intermediate deoxyhypusine residue. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DHS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 70-369 mapping at the C-terminus of DHS of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 70-369 mapping at the C-terminus of DHS of human origin |
| Entrez Gene | 1725 |
|---|
Application
Western blot analysis of DHS expression in non-transfected: (A) and human DHS transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of DHS expression in HeLa whole cell lysate.| Application Notes | recommended for detection of DHS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
