Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of cells in seminiferous ducts.Application
Western blot analysis of SPT5 expression in non-transfected: (A) and mouse SPT5 transfected: (B) 293T whole cell lysates and DU 145 nuclear extract (C).Application
Western blot analysis of SPT5 expression in CCRF-CEM (A) and DU 145 (B) nuclear extracts.| Product Name | SPT5 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | SPT4 (also designated Suppressor of Ty4 and p14) and SPT5 (also designated DSIF p160) are highly conserved proteins from yeast to humans (1). Nuclear SPT4 and SPT5 are involved in both DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole)-mediated transcriptional inhibition as well as the activation of transcriptional elongation by the HIV-1 protein Tat. SPT4 binds SPT5 to form the DSIF (DRB-sensitivity-inducing factor) complex, which binds RNA polymerase II and directly regulates elongation. However, SPT5 protein in mitotic HeLa cells migrates more slowly on SDS-PAGE than does SPT5 isolated from interphase cells, as a result of enhanced SPT5 phosphorylation. The C-terminal CTR1 domain of SPT5 is the substrate for P-TEFb phosphorylation, which is critical for SPT5 function as a regulator of transcriptional elongation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of SPT5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 61-360 mapping near the N-terminus of SPT5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 61-360 mapping near the N-terminus of SPT5 of human origin |
| Entrez Gene | 6829 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of cells in seminiferous ducts.
Application
Western blot analysis of SPT5 expression in non-transfected: (A) and mouse SPT5 transfected: (B) 293T whole cell lysates and DU 145 nuclear extract (C).
Application
Western blot analysis of SPT5 expression in CCRF-CEM (A) and DU 145 (B) nuclear extracts.| Application Notes | recommended for detection of SPT5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
