Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing nuclear envelope, nuclear and cytoplasmic staining of glandular cells.Application
Western blot analysis of LAP2 expression in non-transfected: (A) and human LAP2 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervical cancer tissue showing perinuclear staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of human recombinant LAP2 fusion protein.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear membrane localization.| Product Name | LAP2 Antibody (H-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The nuclear envelope separates the nucleoplasm from the cytoplasm in eukaryotic cells and includes the outer and inner nuclear membrane, nuclear pore complexes and the nuclear lamina. The nuclear lamina contains intermediate filament-type proteins called lamins that form a dense network to strengthen and stabilize the nuclear envelope. Lamina-associated polypeptide 2 (LAP2) is also known as thymopoietin. LAP2 is a nuclear envelope protein and contains an amino-terminal region called the LAP2-emerin-MAN1 or LEM motif. LAP2 also contains a unique DNA-binding amino-terminal domain. Alternative splicing produces six isoforms (a–g) of mammalian LAP2 and three isoforms in Xenopus LAP2. LAP2a and LAP2b associate with chromosomal barrier-to-autointegration factor (BAF) and may play a role in stabilizing chromatin structure. LAP2b also binds to lamin B. LAP2a is a non-membrane isoform of LAP2 that associates with the internal nucleoskeleton and binds lamin A. The gene encoding human LAP2 maps to chromosome 12q23.1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of LAP2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-70 mapping at the N-terminus of LAP2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-70 mapping at the N-terminus of LAP2 of human origin |
| Entrez Gene | 7112 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing nuclear envelope, nuclear and cytoplasmic staining of glandular cells.
Application
Western blot analysis of LAP2 expression in non-transfected: (A) and human LAP2 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervical cancer tissue showing perinuclear staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of human recombinant LAP2 fusion protein.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear membrane localization.| Application Notes | recommended for detection of LAP2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
