Application
Western blot analysis of Ral A expression in non-transfected: (A) and human Ral A transfected: (B) 293T whole cell lysates.| Product Name | Ral A/B Antibody (H-46) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Ral A and Ral B constitute a distinct subfamily of Ras-related GTPases (i.e., GDP/GTP binding proteins) (1-3). Ral proteins are activated by a unique nucleotide exchange factor, Ral GDS, and deactivated by a distinct GTPase-activating protein (4,5). Unlike Ras proteins, Ral A and Ral B fail to induce transformed foci when activated variants are expressed in various recipient cells (1). A potential downstream target of Ral, designated Ral BP-1, has been shown to contain a Rho-GTPase-activating domain (1). This Rho-GTPase-activating domain interacts preferentially with the Rho family member Cdc42 (1). A Ras/Ral signaling pathway has been reported to mediate phospholipase D (PLD) activation by v-Src, thus indicating PLD as another downstream target of Ral A (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ral A and B of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 161-206 mapping at the C-terminus of Ral A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 161-206 mapping at the C-terminus of Ral A of human origin |
| Entrez Gene | 5898 |
|---|
Application
Western blot analysis of Ral A expression in non-transfected: (A) and human Ral A transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Ral A and B of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
