Application
Western blot analysis of SMS1 expression in non-transfected: (A) and mouse SMS1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SMS1 expression in non-transfected: (A) and human SMS1 transfected: (B) 293T whole cell lysates.| Product Name | SMS1 Antibody (H-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The SMS (sphingomyelin synthase) family is a group of integral membrane proteins that includes SMS1 (sphingomyelin synthase 1) and SMS2 (sphingomyelin synthase 2). SMS1 is located in the Golgi apparatus, whereas SMS2 resides primarily at the plasma membrane. Both are bidirectional lipid cholinephosphotransferases which convert phosphatidylcholine (PC) and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) and vice versa, the direction of which depends on the relative concentrations of ceramide and diacylglycerol as phosphocholine acceptors. Therefore, sphingomyelin synthases are thought to be involved in both cell death and survival. Tricyclodecan-9-yl-xanthogenate (D609), a selective tumor cytotoxic agent, inhibits SMS activity, contributing to tumor cell cytotoxicity. SMS proteins are expressed in liver, muscle, heart, brain, stomach and kidney. SMS1 is expressed as four alternatively spliced mRNAs (SMS1å1, SMS1å2, SMS1∫ and SMS1©) that translate into three different proteins (SMS1å, SMS1∫ and SMS1©), which differ in their tissue distribution and function. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SMS1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-150 mapping at the N-terminus of SMS1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 21-150 mapping at the N-terminus of SMS1 of human origin |
| Entrez Gene | 259230 |
|---|
Application
Western blot analysis of SMS1 expression in non-transfected: (A) and mouse SMS1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SMS1 expression in non-transfected: (A) and human SMS1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of SMS1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
