Application
Western blot analysis of Mig-6 expression in non-transfected: (A) and mouse Mig-6 transfected: (B) 293T whole cell lysates.| Product Name | Mig-6 Antibody (H-125) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Mitogen-inducible gene 6 protein (Mig-6), also designated Gene 33 or RALT, belongs to the Mig-6 family. The gene encoding for Mig-6 maps to chromosome 1p36.12-p36.33. Mig-6 is a cytoplasmic protein acting as a feedback inhibitor of ErbB-2 mitogenic function and can suppress ErbB-2 oncogenic activity. The expression of Mig-6 is upregulated with cell growth. Mig-6 binds to the epidermal growth factor receptor (EGFR) upon EGF stimulation and is considered a negative feedback regulator of EGFR and a potential tumor suppressor. Mig-6 induces transcriptional activation of NF˚B by binding to its inhibitor I˚B-å. It enables the cell to respond persistently to chronic stress. Mig-6 mRNA levels increase in response to stress such as diabetic nephropathy, vasoactive peptides or mechanical strain. Mig-6 is expressed in liver, placenta and lung. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Mig-6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 156-280 mapping within an internal region of Mig-6 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 156-280 mapping within an internal region of Mig-6 of human origin |
| Entrez Gene | 54206 |
|---|
Application
Western blot analysis of Mig-6 expression in non-transfected: (A) and mouse Mig-6 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Mig-6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
