Application
Western blot analysis of PDEF expression in non-transfected: (A) and human PDEF transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PDEF expression in non-transfected: (A) and mouse PDEF transfected: (B) 293T whole cell lysates.| Product Name | PDEF Antibody (H-250) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Prostate epithelium-specific Ets transcription factor (PDEF), also designated prostate Ets or SAM pointed domain containing Ets transcription factor, is a 335 amino acid nuclear protein. PDEF belongs to the Ets family of proteins. This protein, which localizes to prostate epithelial cells, functions as an Ets transcription factor. It upregulates the activity of the p62 promotor but this activity can be downregulated by PSI. It is also involved in the activation of prostate-specific antigen (PSA) by acting as an androgen-independent transactivator. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of PDEF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-250 mapping at the N-terminus of PDEF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-250 mapping at the N-terminus of PDEF of human origin |
| Entrez Gene | 25803 |
|---|
Application
Western blot analysis of PDEF expression in non-transfected: (A) and human PDEF transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PDEF expression in non-transfected: (A) and mouse PDEF transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of PDEF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
