Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of INSIG-2 expression in Hep G2 whole cell lysate.Application
Western blot analysis of INSIG-2 expression in mouse liver tissue extract.| Product Name | INSIG-2 Antibody (H-40) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | INSIG-1 and INSIG-2 play distinct roles in a negative-feedback mechanism for cholesterol synthesis. INSIG-1 localizes to the endoplasmic reticulum (ER) and binds the sterol-sensing domain of SREBP cleavage-activating protein (SCAP). Sterol induces INSIG-1 binding to SCAP. INSIG-2, another ER protein, binds SCAP in a sterol-regulated manner. Thus, INSIG-1 and INSIG-2 block the export of SCAP from the ER and ultimately inhibit cholesterol synthesis by preventing the proteolytic processing of SREBPs by Golgi enzymes. The critical role of INSIG-1 and INSIG-2 in cholesterol metabolism may be exploited as a therapeutic effect for hypercholesterolemia. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of INSIG-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-40 mapping at the N-terminus of INSIG-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-40 mapping at the N-terminus of INSIG-2 of human origin |
| Entrez Gene | 51141 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of INSIG-2 expression in Hep G2 whole cell lysate.
Application
Western blot analysis of INSIG-2 expression in mouse liver tissue extract.| Application Notes | recommended for detection of INSIG-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
