Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of Leydig cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colo-rectal cancer tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of IGF-IIR expression in HeLa whole cell lysate.| Product Name | IGF-IIR Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The mannose 6-phosphate/insulin-like growth factor II receptor, IGF-IIR (also designated M6P/IGF2R), is a ubiquitously expressed integral glycoprotein. By binding glycoproteins through two of its extracytoplasmic domains, IGF-IIR mediates the activation of TGF∫1 (a growth inhibitor), the degradation of IGF-II and the transport of lysosomal enzymes. Subsequently, IGF-IIR can form oligomeric complexes, which increase the affinity of IGF-IIR for lysosomal enzymes. Unlike IGF-IR, IGF-IIR does not potentiate the signaling of IGF-I or IGF-II, which have mitogenic, cell survival and insulin-like effects. Therefore, IGF-IIR is characterized as a tumor suppressor. Furthermore, the IGF-IIR gene is located on chromosome 6q26, which is commonly mutated or deleted in several human cancers. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of IGF-IIR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 2192-2491 of IGF-IIR of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 2192-2491 of IGF-IIR of human origin |
| Entrez Gene | 3482 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of Leydig cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colo-rectal cancer tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of IGF-IIR expression in HeLa whole cell lysate.| Application Notes | recommended for detection of IGF-IIR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
