Application
Western blot analysis of mouse recombinant His-probe tagged GRIP1 (A) and His-probe tagged IKKα (B) fusion protein.Application
Western blot analysis of mouse recombinant His-probe-tagged GRIP1.Application
Western blot analysis of Mad 1 (1-221): WB (A) and PAR4 (1-334): WB (B) polyhistidine tagged fusion proteins using primary antibody at 0.1 µg/ml.| Product Name | His-probe Antibody (H-15) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of fusion proteins encoded by prokaryotic pET, pRSET A, B, C and pTrc His A, B, C baculovirus pBlue Bac His A, B, C and mammalian pEBV His A, B, C expression vectors by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping to the polyhistidine domains of pET and Xpress polyhistidine expression vectors |
|---|---|
| Tissue Specificity | epitope mapping to the polyhistidine domains of pET and Xpress polyhistidine expression vectors |
Application
Western blot analysis of mouse recombinant His-probe tagged GRIP1 (A) and His-probe tagged IKKα (B) fusion protein.
Application
Western blot analysis of mouse recombinant His-probe-tagged GRIP1.
Application
Western blot analysis of Mad 1 (1-221): WB (A) and PAR4 (1-334): WB (B) polyhistidine tagged fusion proteins using primary antibody at 0.1 µg/ml.| Application Notes | recommended for detection of fusion proteins encoded by prokaryotic pET, pRSET A, B, C and pTrc His A, B, C baculovirus pBlue Bac His A, B, C and mammalian pEBV His A, B, C expression vectors by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
