Application
Western blot analysis of CD100 expression in Jurkat whole cell lysate.Application
Western blot analysis of CD100 expression in non-transfected: (A) and human CD100 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CD100 expression in SK-N-SH (A) and WEHI-231 (B) whole cell lysates.| Product Name | CD100 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Semaphorins comprise a family of phylogenetically conserved proteins containing a 500 amino acid region termed the “Sema domain” in the amino-terminus. The semaphorin family is subdivided into eight groups. CD100 (SEMA4D) is a transmembrane protein belonging to group IV of the semaphorin family. CD100 is expressed in both embryonic and adult kidney, heart, lung and neural tissues. Plexin-B1, a receptor expressed abundantly in fetal brain and kidney, binds CD100 with high affinity. CD100 is the only member of the semaphorin family that is expressed in the immune system. CD100 activates T cell proliferation and promotes B cell aggregation. CD100 regulates B cell response by negating an inhibitory signal from CD72 that normally diminishes B cell response. This signal is turned off when CD100 binds to CD72 and induces tyrosine dephosphorylation of CD72, followed by the dissociation of SHP-1 from CD72. The gene encoding human CD100 maps to chromosome 9q22-q31. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CD100 of human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 563-862 of CD100 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 563-862 of CD100 of human origin |
| Entrez Gene | 10507 |
|---|
Application
Western blot analysis of CD100 expression in Jurkat whole cell lysate.
Application
Western blot analysis of CD100 expression in non-transfected: (A) and human CD100 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CD100 expression in SK-N-SH (A) and WEHI-231 (B) whole cell lysates.| Application Notes | recommended for detection of CD100 of human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
