Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic localization.| Product Name | Clathrin HC Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Clathrin is a major cytosolic coat protein in pits and vesicles originating from the plasma membrane and the trans-Golgi network (1,2). In receptor-mediated endocytosis, receptor proteins are englufed by clathrin-coated vesicles (3,4). Clathrin is composed of three heavy chains and three light chains which associate non-covalently to form a triskelion structure (5,6). Clathrin heavy chain (HC) is composed of a terminal globular domain, a distal segment and a proximal segment containing a light chain binding site (7). The proximal segment of the clathrin HC protein is essential for interactions between clathrin heavy chains and light chains which result in the formation of the triskelion structure (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAChIP |
| recommended for detection of Clathrin heavy chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1321-1620 of Clathrin HC 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1321-1620 of Clathrin HC 1 of human origin |
| Entrez Gene | 1213 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic localization.| Application Notes | recommended for detection of Clathrin heavy chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
