Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of transgelin expression in A-10 whole cell lysate.Application
Western blot analysis of transgelin expression in non-transfected 293T: (A), mouse transgelin transfected 293T: (B) and Hs68 (C) whole cell lysates.Application
Western blot analysis of transgelin expression in Hs68 (A) and WI 38 (B) whole cell lysates.| Product Name | transgelin Antibody (H-75) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Transgelin, also designated SM22a, is expressed abundantly in smooth muscle cells. The human transgelin gene (designated TAGLN), which is located on chromosome 11q23.3, encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is downregulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of transgelin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 16-90 mapping near the N-terminus of transgelin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 16-90 mapping near the N-terminus of transgelin of human origin |
| Entrez Gene | 6876 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of transgelin expression in A-10 whole cell lysate.
Application
Western blot analysis of transgelin expression in non-transfected 293T: (A), mouse transgelin transfected 293T: (B) and Hs68 (C) whole cell lysates.
Application
Western blot analysis of transgelin expression in Hs68 (A) and WI 38 (B) whole cell lysates.| Application Notes | recommended for detection of transgelin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
