Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of Trx-2 expression in HeLa whole cell lysate.Application
Western blot analysis of Trx-2 expression in non-transfected: (A) and human Trx-2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Trx-2 expression in non-transfected: (A) and human Trx-2 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | Trx-2 Antibody (H-75) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Thioredoxin (Trx) is a redox protein found in several species, such as bacteria, plants and mammals, and contains a conserved active site consisting of Trp-Cys-Gly-Pro-Cys. Trx has several biological functions. It acts as a hydrogen donor for ribonucleotide reductase, which is critical for DNA synthesis, and modulates the DNA-binding activity of several transcription factors, including NFkB, AP-1, p53, TFIIIC and glucocorticoid receptor. Trx also stimulates cell growth, is an inhibitor of apoptosis and plays a role in the protection against oxidative stress. Drugs that inhibit Trx have antitumor activity, suggesting that Trx is involved in a variety of human diseases, including cancer. Thioredoxin 2 (Trx-2) is a small redox protein that is localized to the mitochondria and is essential for cell viability, playing a crucial role in the scavenging of ROS in mitochondria and regulating the mitochondrial apoptosis signaling pathway. Trx reductases (TrxR1 and TrxR2) are ubiquitously expressed flavoproteins that catalyze the NADPH-dependent reduction of Trx as well as several other oxidized cellular components. Mammalian Trx reductases are a part of a selenium-containing pyridine nucleotide-disulphide oxidoreductase family, which has a conserved catalytic site of Cys-Val-Asn-Val-Gly-Cys. TrxR1 and TrxR2 are also involved in the prevention of oxidative stress. Inhibition of TrxR activity may provide for potential treatments of cancer, AIDS and other autoimmune diseases as well as bacterial infections and parasitic diseases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Trx-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 92-166 mapping at the C-terminus of Trx-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 92-166 mapping at the C-terminus of Trx-2 of human origin |
| Entrez Gene | 25828 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of Trx-2 expression in HeLa whole cell lysate.
Application
Western blot analysis of Trx-2 expression in non-transfected: (A) and human Trx-2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Trx-2 expression in non-transfected: (A) and human Trx-2 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | recommended for detection of Trx-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
