Application
Western blot analysis of Siva expression in HeLa whole cell lysate.Application
Western blot analysis of Siva expression in non-transfected: (A) and mouse Siva transfected: (B) 293T whole cell lysates.| Product Name | Siva Antibody (M-175) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | A cytoplasmic domain of approximately 80 amino acids was identified in the apoptosis-mediating receptors TNFR1 and Fas. This region was determined to be necessary for the transduction of the apoptotic signal and was designated the “death domain”. Other death domain-containing, but otherwise structurally unrelated, proteins have been identified on the basis of their ability to associate with the cytoplasmic domains of TNFR1 or FAS. FADD (also designated MORT1) and TRADD bind to Fas and TNFR1, respectively. RIP is a death domain-containing serine/threonine kinase that binds to TRADD (5). RAIDD (also designated CRADD) was identified as a RIP binding protein. Both RAIDD and FADD can associate with members of the caspase family, providing a link between the activation of theTNFRs and the triggering of the cysteine protease cascade. The death domain-containing protein SIVA binds to the TNFR family member CD27 and appears to play a role in CD27 mediated apoptosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Siva of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-175 representing full length Siva of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-175 representing full length Siva of mouse origin |
| Entrez Gene | 10572 |
|---|
Application
Western blot analysis of Siva expression in HeLa whole cell lysate.
Application
Western blot analysis of Siva expression in non-transfected: (A) and mouse Siva transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Siva of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
