Application
Western blot analysis of Gα t1 expression in non-transfected: (A) and mouse Gα t1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Gα t1 expression in rat brain tissue extract.Application
Western blot analysis of Gα t1 expression in rat (A) and cow (B) brain extracts.| Product Name | Gα t1 Antibody (K-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors (1). Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i.e., adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein å, ∫ and © polypeptides are encoded by at least 16, 4 and 7 genes, respectively (2-5). Most interest in G proteins has been focused on their å subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of Ga subunits have been identified; these include Gs, Gi, Gq and Gå 12/13 (3,4). The Gi class comprises all the known a subunits that are susceptible to pertussis toxin modifications, including Gå i-1, Gå i-2, Gå i-3, Gå o, Gå t1, Gå t2, Gå z and Gå gust (4). In the well characterized visual system, photorhodopsin catalyzes the exchange of guanine nucleotides bound to the visual transducin Gå subunits (Gå ti in rod cells and Gå t2 in cone cells) (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of G&alpha t1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within a highly divergent domain of G&alpha t1(G&alpha rod transducin) of human origin |
|---|---|
| Tissue Specificity | epitope mapping within a highly divergent domain of Gαt1(Gαrod transducin) of human origin |
| Entrez Gene | 2779 |
|---|
Application
Western blot analysis of Gα t1 expression in non-transfected: (A) and mouse Gα t1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Gα t1 expression in rat brain tissue extract.
Application
Western blot analysis of Gα t1 expression in rat (A) and cow (B) brain extracts.| Application Notes | recommended for detection of G&alpha t1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
