Application
Western blot analysis of NSF expression in SK-N-MC (A), MIA PaCa-2 (B) and EOC 20 (C) whole cell lysates.Application
Western blot analysis of NSF expression in non-transfected: (A) and mouse NSF transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate cancer tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing cytoplasmic localization.Application
Western blot analysis of NSF expression in mouse brain extract.| Product Name | NSF Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimide-sensitive factor), SNAP 25 (synaptosomal-associated protein of 25kDa), SNAPs (soluble NSF attachment proteins) and synaptotagmin. VAMPs, also designated synaptobrevins, including VAMP-1 and VAMP-2, and synaptotagmin, a protein that may function as an inhibitor of exocytosis, are vesicular proteins. SNAPs, including a- and g-SNAP, are cytoplasmic proteins that bind to a membrane receptor complex composed of VAMP, SNAP 25 and syntaxin. SNAPs mediate the membrane binding of NSF, which is essential for membrane fusion reactions. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of NSF and LOC728806 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of NEM-sensitive fusion protein (NSF) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of NEM-sensitive fusion protein (NSF) of human origin |
| Entrez Gene | 4905 |
|---|
Application
Western blot analysis of NSF expression in SK-N-MC (A), MIA PaCa-2 (B) and EOC 20 (C) whole cell lysates.
Application
Western blot analysis of NSF expression in non-transfected: (A) and mouse NSF transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate cancer tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing cytoplasmic localization.
Application
Western blot analysis of NSF expression in mouse brain extract.| Application Notes | recommended for detection of NSF and LOC728806 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
