Application
Western blot analysis of Nanog expression in non-transfected: (A) and human Nanog transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Nanog expression in non-transfected: (A) and human Nanog transfected: (B) 293 whole cell lysates.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear localization.| Product Name | Nanog Antibody (M-149) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Nanog (from “Tir Na Nog,” the mythologic Celtic land of the ever young) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression have been detected by Northern analysis in the undifferentiated NTERA-2 cl.D1 embryonal carcinoma cell line. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Nanog of mouse and rat origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 181-329 mapping at the C-terminus of Nanog of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 181-329 mapping at the C-terminus of Nanog of mouse origin |
| Entrez Gene | 71950 |
|---|
Application
Western blot analysis of Nanog expression in non-transfected: (A) and human Nanog transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Nanog expression in non-transfected: (A) and human Nanog transfected: (B) 293 whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear localization.| Application Notes | recommended for detection of Nanog of mouse and rat origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
