Application
Western blot analysis of MP1 expression in non-transfected: (A) and mouse MP1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of MP1 expression in 3T3-L1 whole cell lysate.| Product Name | MP1 Antibody (FL-124) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | MP1 (MEK partner 1) functions as a scaffolding protein in the mitogen activated protein (MAP) kinase signaling pathway. Growth factor induced MAP kinase activation is selectively mediated by the extracellular signal-regulated kinase (ERK) cascade. This pathway is dependent on the phosphorylation of MEK-1 and its subsequent activation of ERK 1. MP1 binds to the proline-rich domain of MEK-1 and thereby potentiates the phosphorylation of MEK-1 by the activating MEK kinase B-Raf. MP1 is also able to enhance the kinase activity of MEK-1 and facilitate the phosphorylation of ERK 1. In vivo studies indicate that MP1 preferentially associates with MEK-1 and ERK 1, but not with MEK-2 or ERK 2, suggesting that MP1 and other scaffolding proteins contribute to the specificity of the kinase substrates within the MAPK pathways. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MP1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-124 representing full length MP1 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-124 representing full length MP1 of mouse origin |
| Entrez Gene | 8649 |
|---|
Application
Western blot analysis of MP1 expression in non-transfected: (A) and mouse MP1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of MP1 expression in 3T3-L1 whole cell lysate.| Application Notes | recommended for detection of MP1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
