Application
Western blot analysis of Annexin II expression in non-transfected 293T: (A), mouse Annexin II transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of Annexin II expression in non-transfected 293T: (A), mouse Annexin II transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of Annexin II expression in non-transfected 293T: (A), mouse Annexin II transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Annexin II expression in non-transfected: (A) and human Annexin II transfected: (B) 293 whole cell lysates.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing membrane localization.Application
Western blot analysis of Annexin II expression in MDCK (A) and NIH/3T3 (B) whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human skin showing membrane localization.| Product Name | Annexin II Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The annexin family of calcium-binding proteins is composed of at least ten mammalian genes and is characterized by a conserved core domain which binds phospholipids in a Ca2+-dependent manner and a unique amino-terminal region which may confer binding specificity. The interaction between these proteins and biological membranes has led to the hypothesis that they are involved in cellular trafficking processes such as endocytosis, exocytosis and cellular adhesion. Annexin I, alternatively referred to as lipocortin, has been implicated as a mediator of the anti-inflammatory response produced by glucocorticoids and as an inhibitor of cPLA2, a potent mediator of inflammation. Annexin II, also called p36, exists as a monomer or as a heterotetramer, complexed with the S-100-related protein p11. This complex is termed calpactin I. In the tetrameric form, Annexin II is an efficient substrate of PKC family and Src pp60. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Annexin II of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-50 of Annexin II of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-50 of Annexin II of human origin |
| Entrez Gene | 302 |
|---|
Application
Western blot analysis of Annexin II expression in non-transfected 293T: (A), mouse Annexin II transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of Annexin II expression in non-transfected 293T: (A), mouse Annexin II transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of Annexin II expression in non-transfected 293T: (A), mouse Annexin II transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Annexin II expression in non-transfected: (A) and human Annexin II transfected: (B) 293 whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing membrane localization.
Application
Western blot analysis of Annexin II expression in MDCK (A) and NIH/3T3 (B) whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human skin showing membrane localization.| Application Notes | recommended for detection of Annexin II of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
