Application
Western blot analysis of CAP expression in non-transfected: (A) and mouse CAP transfected: (B) 293T whole cell lysates.| Product Name | CAP Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | c-Cbl associated protein (CAP), also designated ponsin and SH3P12, interacts with c-Cbl and facilitates the tyrosine phosphorylation of c-Cbl in response to insulin. CAP contains three adjacent Src homology-3 (SH3) domains in the carboxy terminus. It interacts with the focal adhesion kinase p125FAK and co-localizes with Actin stress fibers. CAP is expressed in 3T3-L1 adipocytes, but not in 3T3-L1 or NIH/3T3 fibroblasts. Expression of the CAP gene is stimulated by thiazolidinediones (TZDs) through activation of PPARg. In addition to its interaction with c-Cbl, CAP interacts with Sos through the same SH3 domain, and may facilitate protein-protein associations involved in cell structural changes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CAP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 of CAP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 of CAP of human origin |
| Entrez Gene | 10580 |
|---|
Application
Western blot analysis of CAP expression in non-transfected: (A) and mouse CAP transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of CAP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
