Application
Western blot analysis of yeast recombinant Kss1 fusion protein.| Product Name | Kss1 Antibody (y-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 261-310 mapping near the C-terminus of Kss1 of Saccharomyces cerevisiae origin |
| Modification Notes | Map kinase cascades, consisting of a mitogen-activated protein kinase (also called Erk, for extracellular regulated kinase) and one or more upstream regulatory kinases (MAPKKs), play an integral role in signal transduction. The best characterized MAP kinase pathway to date is the yeast pheromone response pathway (1). Extracellular pheromones bind to the receptors Ste2 and Ste3 on the cell surface. Activation of these receptors eventually leads to stimulation of the MAPKKK Ste11 (2). Upon phosphorylation, Ste11 activates the MAPKK Ste7 (3), which in turn activates the MAP kinases Fus3 (also called Dac2) and Kss1 (4,5). These MAP kinases activate the transcription factor Ste12, which upregulates mating-specific genes (6,7), and Far1, which arrests the cell cycle (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Kss1 of Saccaromyces cerevisiae origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 261-310 mapping near the C-terminus of Kss1 of Saccharomyces cerevisiae origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 261-310 mapping near the C-terminus of Kss1 of Saccharomyces cerevisiae origin |
Application
Western blot analysis of yeast recombinant Kss1 fusion protein.| Application Notes | recommended for detection of Kss1 of Saccaromyces cerevisiae origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
