Application
Western blot analysis of ACOT8 expression in non-transfected 293T: (A), mouse ACOT8 transfected 293T: (B) and Ramos (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of human recombinant ACOT8.| Product Name | ACOT8 Antibody (H-320) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | ACOT8 (Acyl-CoA thioesterase 8), also designated TEII p35 or hTE, is a novel human thioesterase known to interact with the HIV protein Nef using yeast two-hybrid screening. Nef is an auxiliary gene of the human immunodeficiency virus (HIV) which facilitates virus replication and enhances infectivity. The roles of Nef in HIV-infected cells are likely mediated by specific interactions with cellular proteins. The interaction between Nef and ACOT8 is correlated with CD4 downregulation, suggesting that ACOT8 may be involved in Nef-mediated CD4 downregulation in HIV-infected cells. ACOT8 is 42% identical to thioesterase II from Escherichia coli, and has no significant homology with the two types of animal thioesterases previously been cloned (type I and type II thioesterases). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of ACOT8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-320 representing full length ACOT8 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-320 representing full length ACOT8 of human origin |
| Entrez Gene | 10005 |
|---|
Application
Western blot analysis of ACOT8 expression in non-transfected 293T: (A), mouse ACOT8 transfected 293T: (B) and Ramos (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of human recombinant ACOT8.| Application Notes | recommended for detection of ACOT8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
