Application
Western blot analysis of phosphorylated PKB Kinase expression in untreated (A) and EGF-treated (B) MDA-MB-435 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Product Name | p-PKB Kinase Antibody (Ser 241) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 241 of PKB Kinase of human origin |
| Modification | Phosphoralated |
| Modification Notes | Two serine/threonine kinases, designated Akt1 and Akt2 (alternatively referred to as PKB or RacPK), exhibit sequence homology with the protein kinase A and C families and are encoded by the c-akt proto-oncogene. Akt1 and Akt2 contain a pleckstrin homology domain (PH) and are activated by PDGF stimulation. This activation is dependent on PDGFR-∫ tyrosine residues 740 and 751, which bind a subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Additionally, the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin. Taken together, these data strongly suggests that the protein signals downstream of the PI kinases. PKB kinase (for protein kinase B kinase) has also been identified as a primary signal transducer that enables the activation of Akt by the PI 3-kinase complex. Phosphorylation of Ser-241 is essential for PKB Kinase activity, unlike its other phosphorylation sites. PKB Kinase can phosphorylate itself at Ser-241, leading to its own activation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 241 phosphorylated PKB Kinase of human origin and correspondingly phosphorylated Ser 244 of mouse and rat origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 241 of PKB Kinase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 241 of PKB Kinase of human origin |
| Entrez Gene | 5170 |
|---|
Application
Western blot analysis of phosphorylated PKB Kinase expression in untreated (A) and EGF-treated (B) MDA-MB-435 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Application Notes | recommended for detection of Ser 241 phosphorylated PKB Kinase of human origin and correspondingly phosphorylated Ser 244 of mouse and rat origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
