Application
Western blot analysis of TOB1 expression in mouse brain tissue extract.Application
Western blot analysis of TOB1 expression in 293T whole cell lysate.| Product Name | TOB1 Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 271-340 mapping near the C-terminus of TOB1 of human origin |
| Modification Notes | TOB1 (TROB, APRO6, PIG49) and TOB2 (TOB4, TROB2, TOBL) are anti-proliferative proteins that modulate cell cycle progression from the G0/G1 to S phases through interactions with the mammalian homologue of yeast Caf1. TOB proteins present in the central nervous system may be engaged in acquisition of motor skill. TOB1 in T lymphocytes can interact with Smad2/4, augment SMAD DNA binding to the IL-2 promoter, and lead to an inhibition of IL-2 transcription. In oncogenic ErbB-2-transformed cells, nuclear export of TOB1 results in a decrease in antiproliferative activity. ERK/MAPK (ERK2) and JNK/SAPK (JNK2) phosphorylate TOB1 in vitro, and TOB1 can undergo phosphorylation at Ser 152, Ser 154 and Ser 164 by ERK1/2 upon growth-factor stimulation. TOB2 gene encodes a 4.1-kb transcript with high expression in skeletal muscle, thymus and ovary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TOB1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 271-340 mapping near the C-terminus of TOB1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 271-340 mapping near the C-terminus of TOB1 of human origin |
| Entrez Gene | 10140 |
|---|
Application
Western blot analysis of TOB1 expression in mouse brain tissue extract.
Application
Western blot analysis of TOB1 expression in 293T whole cell lysate.| Application Notes | recommended for detection of TOB1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
