Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear and cytoplasmic staining of squamous epithelial cells.Application
Western blot analysis of HAUSP expression in HeLa (A), A-431 (B), K-562 (C) and Jurkat (D) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of HAUSP expression in HeLa (A) and A-431 (B) whole cell lysates.| Product Name | HAUSP Antibody (H-200) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 11-210 mapping near the N-terminus of HAUSP of human origin |
| Modification Notes | HAUSP (herpesvirus-associated ubiquitin-specific protease, USP7) is a ubiquitin-specific protease. HAUSP localizes predominantly to the nucleus, in a TD-dependent manner, where it associates with ND10 (1,2). ND10 are small nuclear structures implicated in a variety of cellular processes including response to stress and interferons, oncogenesis, and viral infection (2). HAUSP binds strongly to Vmw110, a herpesvirus regulatory protein which has the ability to disrupt ND10 (2,3). HAUSP, a novel p53-interacting protein, functions to deubiquitinize p53 in an important pathway for p53 stabilization (4,5). HAUSP strongly stabilizes p53 even in the presence of excess Mdm2, and also induces p53-dependent cell growth repression and apoptosis (4). The HAUSP protein is distributed in the nucleus in a micropunctate pattern with a limited number of larger discrete foci, some of which co-localize with PML in ND10 (6). The gene encoding HAUSP maps to human chromosome band 16p13.2 (5,6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of HAUSP of mouse, rat and human origin by WB, IP, IF, IHC(E) and ELISA also reactive with additional species, including canine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 11-210 mapping near the N-terminus of HAUSP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 11-210 mapping near the N-terminus of HAUSP of human origin |
| Entrez Gene | 7874 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear and cytoplasmic staining of squamous epithelial cells.
Application
Western blot analysis of HAUSP expression in HeLa (A), A-431 (B), K-562 (C) and Jurkat (D) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of HAUSP expression in HeLa (A) and A-431 (B) whole cell lysates.| Application Notes | recommended for detection of HAUSP of mouse, rat and human origin by WB, IP, IF, IHC(E) and ELISA also reactive with additional species, including canine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
