Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.Application
Western blot analysis of TF expression in non-transfected: (A) and mouse TF transfected: (B) 293T whole cell lysates.| Product Name | TF Antibody (FL-294) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII), which are involved in a blood coagulation cascade leading to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Coagulation Factor V (Factor V, FV, proaccelerin, labile factor) is a 2196 amino acid, single chain glycoprotein that is cleaved by Thrombin to yield an active, Ca2+-dependent dimer that is essential to the blood coagulation cascade. Together with catalytic Factor Xa and Ca2+ on the surface of platelets or endothelial cells, Factor Va coordinates into a Prothrombinase complex, which mediates proteolysis of Prothrombin into active Thrombin. Tissue factor (TF), also designated coagulation Factor III) is a cell surface glycoprotein that enables cells to initiate blood coagulation cascades. It functions as a high-affinity receptor for coagulation Factor VII. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of tissue factor (TF) of mouse, rat and, to a lesser extent, human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-294 representing full length TF of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-294 representing full length TF of mouse origin |
| Entrez Gene | 2152 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Western blot analysis of TF expression in non-transfected: (A) and mouse TF transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of tissue factor (TF) of mouse, rat and, to a lesser extent, human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
