Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of PYK2 expression in Ramos whole cell lysate.Application
Western blot analysis of PYK2 expression in non-transfected 293T: (A), human PYK2 transfected 293T: (B) and Ramos (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic staining.Application
Western blot analysis of PYK2 expression in PC-12 (A) and Ramos (B) whole cell lysates.| Product Name | PYK2 Antibody (H-102) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Focal adhesion kinase (FAK) was initially identified as a substrate for the intrinsic protein tyrosine kinase activity of Src-encoded pp60. The deduced amino acid sequence of FAK p125 has shown it to be a cytoplasmic protein tyrosine kinase whose sequence and structural organization are unique compared to other protein families described. A putative new member of the FAK family, designated PYK2 (proline-rich tyrosine kinase 2), exhibits 61% sequence identity with FAK over its kinase domain. PYK2 (also designated CAKß or RAFTK) is highly expressed in the central nervous system. Activation of the kinase leads to modulation of ion channel function and the activation of the MAPK signaling pathway. PYK2 is rapidly phosphorylated on tyrosine residues in response to stimuli that increase intracellular calcium levels and compounds that activate members of the PKC family of kinases, such as phorbol esters. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PYK2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-102 mapping at the N-terminus of PYK2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-102 mapping at the N-terminus of PYK2 of human origin |
| Entrez Gene | 2185 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of PYK2 expression in Ramos whole cell lysate.
Application
Western blot analysis of PYK2 expression in non-transfected 293T: (A), human PYK2 transfected 293T: (B) and Ramos (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic staining.
Application
Western blot analysis of PYK2 expression in PC-12 (A) and Ramos (B) whole cell lysates.| Application Notes | recommended for detection of PYK2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
