Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing cytoplasmic and nuclear staining of respiratory epithelial cells.Application
Antibodies tested include PI 3-kinase p110β (S-19): (A) and PI 3-kinase p110β (N-20): (B).| Product Name | PI 3-kinase p110β Antibody (S-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of PI 3-kinase p110β of human origin |
| Modification Notes | Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85å and p85∫), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110å and p110∫ interact with p85å, and p110å has also been shown to interact with p85∫ in vitro. p110∂ expression is restricted to white blood cells. It has been shown to bind p85å and ∫, but it apparently does not phosphorylate these subunits. p110∂ seems to have the capacity to autophosphorylate. p110© does not interact with the p85 subunits. It has been shown to be activated by å and ∫© heterotrimeric G proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PI 3-kinase p110β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of PI 3-kinase p110&beta of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of PI 3-kinase p110β of human origin |
| Entrez Gene | 5291 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing cytoplasmic and nuclear staining of respiratory epithelial cells.
Application
Antibodies tested include PI 3-kinase p110β (S-19): (A) and PI 3-kinase p110β (N-20): (B).| Application Notes | recommended for detection of PI 3-kinase p110β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
