Application
Western blot analysis of MSK1 expression in HL-60 (A), HeLa (B) and THP-1 (C) whole cell lysates.Application
Western blot analysis of MSK1 expression in UV-induced HeLa (A) and JAR (B) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tumor showing nuclear localization.| Product Name | MSK1 Antibody (H-65) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The family of ribosomal S6 kinases (Rsks), designated Rsk-1, Rsk-2 and Rsk-3, have been implicated as important signaling intermediates in response to a broad range of ligand-activated receptor tyrosine kinases. A unique feature common to the three members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. A related S6 kinase, p70 S6 kinase, functions to phosphorylate the S6 protein on ribosomal 40S subunits. p70 S6 kinase b shares high sequence homology with p70 S6 kinase, except in the carboxy terminus where it contains a proline-rich domain that may be involved in SH3 domain containing protein interactions. MSK1 (also designated RLPK) is related to Rsk and p70 S6 kinase family members and is thought to be structurally similar to Rsk family members, but it may be regulated by distinct mechanisms. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of MSK1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 745-809 mapping at the C-terminus of MSK1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 745-809 mapping at the C-terminus of MSK1 of human origin |
| Entrez Gene | 9252 |
|---|
Application
Western blot analysis of MSK1 expression in HL-60 (A), HeLa (B) and THP-1 (C) whole cell lysates.
Application
Western blot analysis of MSK1 expression in UV-induced HeLa (A) and JAR (B) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tumor showing nuclear localization.| Application Notes | recommended for detection of MSK1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
