Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human soft tissue showing extracellular staining around fibrous and endothelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Fibulin-2 expression in non-transfected 293T: (A), mouse Fibulin-2 transfected 293T: (B) and CCD-1064Sk (C) whole cell lysates.Application
Western blot analysis of Fibulin-2 expression in JEG-3 (A) and CCD-1064Sk (B) whole cell lysates.| Product Name | Fibulin-2 Antibody (H-250) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 28-277 mapping near the N-terminus of Filbulin-2 of human origin |
| Modification Notes | Fibulin-1 and Fibulin-2 associate with Fibronectin and other extracellular matrix proteins. In bone marrow, Fibulin-1 and Fibulin-2 bind to Fibronectin in the adherent layer. Fibulin-1 expression is stimulated by estrogen in ovarian cancer cell lines and has been suggested as both an agent of metastasis in ovarian cancer cells and as an indicator for predicting cancer risk or aggressiveness in ovarian carcinomas. The mobility of cancer cells may be inhibited with increasing exposure to Fibulin-1. Fibulin-2 binds to the lectin domains of extracellular matrix proteins aggrecan, versican and brevican. Fibulin-2 is abundantly expressed in heart, placenta and ovarian tissue, where it localizes to basement membranes and connective tissue compartments. In mice, differential Fibulin-2 gene expression correlates with the early phase of diabetic kidneys and glomerulosclerosis. The gene encoding human Fibulin-2 maps to chromosome 3p25.1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of precursor and mature Fibulin-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 28-277 mapping near the N-terminus of Filbulin-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 28-277 mapping near the N-terminus of Filbulin-2 of human origin |
| Entrez Gene | 2199 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human soft tissue showing extracellular staining around fibrous and endothelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Fibulin-2 expression in non-transfected 293T: (A), mouse Fibulin-2 transfected 293T: (B) and CCD-1064Sk (C) whole cell lysates.
Application
Western blot analysis of Fibulin-2 expression in JEG-3 (A) and CCD-1064Sk (B) whole cell lysates.| Application Notes | recommended for detection of precursor and mature Fibulin-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
