Application
Western blot analysis of Oct-1 expression in WEHI-231 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human tonsil (A) and immunofluorescence staining of methanol-fixed Jurkat cells (B) showing nuclear localization.Application
Western blot analysis of Oct-1 expression in Jurkat nuclear extract.| Product Name | Oct-1 Antibody (C-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Oct-1 of human origin |
| Modification Notes | POU domain proteins contain a bipartite DNA binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer from the Oct-1 gene formed on the palindromic Oct factor-recognition element (PORE), which is comprised of an inverted pair of homeodomain-binding sites separated by exactly 5 bp (ATTTGAAATGCAAAT), could recruit the transcriptional co-activator OBF1. Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, ATTTGCAT, and the proteins that bind to it. This is a regulatory element important for tissue- and cell-specific transcription, as well as for transcription of a number of housekeeping genes. The Oct-1 gene encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by the Oct-2 gene. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Oct-1 of mouse, rat, human and Xenopus origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Oct-1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Oct-1 of human origin |
| Entrez Gene | 5451 |
|---|
Application
Western blot analysis of Oct-1 expression in WEHI-231 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human tonsil (A) and immunofluorescence staining of methanol-fixed Jurkat cells (B) showing nuclear localization.
Application
Western blot analysis of Oct-1 expression in Jurkat nuclear extract.| Application Notes | recommended for detection of Oct-1 of mouse, rat, human and Xenopus origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
