Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic and membrane staining of urothelial cells.Application
Western blot analysis of QM expression in MIA PaCa-2 (A) and PC-3 (B) whole cell lysates.Application
Western blot analysis of QM expression in HeLa whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of cells in red and white pulps at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of QM expression in non-transfected control (A) and QM siRNA transfected (B) HeLa cells. Blot probed with QM (C-17): . GAPDH (FL-335): used as specificity and loading control.Application
Western blot analysis of QM expression in Jurkat (A) and K-562 (B) whole cell lysates and Jurkat (C) and K-562 (D) nuclear extracts.| Product Name | QM Antibody (C-17) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of QM of human origin (differs from corresponding mouse sequence by a single amino acid) |
| Modification Notes | The c-Jun protein is a major component of the transcription factor AP-1 (1,2), originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA-response element (TRE) (1,2). The Jun proteins form homo- and heterodimers which bind the TRE, while Fos proteins are active only as heterodimers with any of the Jun proteins (3,4). Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. A distant member of the MAP kinase family, designated c-Jun NH2-terminal kinase (JNK1) functions to regulate c-Jun by phosphorylation at the amino terminal serine regulatory sites, Ser-63 and Ser-73 (5,6). QM has been described as a transcription factor that can function to bind DNA directly or alternatively can interact with c-Jun to inhibit transactivation of AP-1 promoter driven reporter vectors by Jun-Jun homodimers. QM is highly conserved throughout eukaryotic evolution and is apparently a member of a multi-gene family (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of QM of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of QM of human origin (differs from corresponding mouse sequence by a single amino acid) |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of QM of human origin (differs from corresponding mouse sequence by a single amino acid) |
| Entrez Gene | 6134 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic and membrane staining of urothelial cells.
Application
Western blot analysis of QM expression in MIA PaCa-2 (A) and PC-3 (B) whole cell lysates.
Application
Western blot analysis of QM expression in HeLa whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of cells in red and white pulps at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of QM expression in non-transfected control (A) and QM siRNA transfected (B) HeLa cells. Blot probed with QM (C-17): . GAPDH (FL-335): used as specificity and loading control.
Application
Western blot analysis of QM expression in Jurkat (A) and K-562 (B) whole cell lysates and Jurkat (C) and K-562 (D) nuclear extracts.| Application Notes | recommended for detection of QM of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
