Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of cells in red pulp.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Western blot analysis of FLAP expression in LADMAC whole cell lysate.WB
(A) Representative immunoblots and representative quantitative analyses of PPARγ, α-SMA, Smemb and FLAP (*P<0.05 versus Sham group).WB
(A) Representative immunoblots and representative quantitative analysis for α-SMA, Smemb, PPARγ and FLAP.| Product Name | FLAP Antibody (FL-161) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Arachidonate 5-lipoxygenase-activating protein (FLAP) is an arachidonic acid binding protein that is critical in the biosynthesis of leukotrienes (1). FLAP is an integral membrane protein that catalyzes the transformation of arachidonic acid to leukotriene A4 (2,3). Leukotrienes are the biologically active metabolites of arachidonic acid that are involved in host defense pathways and play an important role in inflammatory diseases like asthma, inflammatory bowel disease, psoriasis and arthritis (2-4). Inhibitors of FLAP function prevent translocation of 5-lipoxygenase from the cytosol to the membrane and inhibit 5-lipoxygenase activation (3). The human FLAP gene, which maps to chromosome 13q12, encodes a 161 amino acid protein (1,3,5). In alveolar macrophages treated with LPS, FLAP activity is suppressed by the inhibition by nitric oxide synthase, although there is no observable decrease in FLAP expression by this pathway (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-161 representing full length FLAP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-161 representing full length FLAP of human origin |
| Entrez Gene | 241 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of cells in red pulp.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Western blot analysis of FLAP expression in LADMAC whole cell lysate.
WB
(A) Representative immunoblots and representative quantitative analyses of PPARγ, α-SMA, Smemb and FLAP (*P<0.05 versus Sham group).
WB
(A) Representative immunoblots and representative quantitative analysis for α-SMA, Smemb, PPARγ and FLAP.| Application Notes | WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
