Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.Application
Western blot analysis of E2F-1 expression in non-transfected: (A) and mouse E2F-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of E2F-1 expression in non-transfected: (A) and human E2F-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of E2F-1 expression in non-transfected: (A) and human E2F-1 transfected: (B) 293T whole cell lysates.Application
Antibodies tested include E2F-1 (C-20): (A), E2F-2 (C-20): (B), E2F-3 (C-18): , E2F-3 (N-20): (C) and E2F-4 (C-20): (D). Data kindly provided by Dr. J. H. J. Petrini and reproduced from Master, et al., 2001. Mol. Cell. Biol. 21: 6006-6016.Application
Controls: normal rabbit IgG: (G), no antibody (H), input DNA (I) and mock (J). Data kindly provided by J. L. Lavrrar and reproduced from J. Biol. Chem. 279: 46343-46349, 2004, with permission from the American Society for Biochemistry and Molecular Biology.Application
Antibodies tested include E2F-1 (C-20): (A), E2F-2 (C-20): (B), E2F-3 (C-18): , E2F-3 (N-20): (C) and E2F-4 (C-20): (D). Data kindly provided by Dr. J. H. J. Petrini and reproduced with permission from Master, et al. 2001. Mol. Cell. Biol. 21: 6006-6016.Application
Western blot analysis of BALB/3T3 cells transfected with either y-CRE (A) or y-CRE/E2F (B).| Product Name | E2F-1 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of E2F-1 of human origin |
| Modification Notes | The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an “active” E2F transcriptional regulatory complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of E2F-1 and, to a lesser extent, E2F-2 and E2F-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of E2F-1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of E2F-1 of human origin |
| Entrez Gene | 1869 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
Application
Western blot analysis of E2F-1 expression in non-transfected: (A) and mouse E2F-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of E2F-1 expression in non-transfected: (A) and human E2F-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of E2F-1 expression in non-transfected: (A) and human E2F-1 transfected: (B) 293T whole cell lysates.
Application
Antibodies tested include E2F-1 (C-20): (A), E2F-2 (C-20): (B), E2F-3 (C-18): , E2F-3 (N-20): (C) and E2F-4 (C-20): (D). Data kindly provided by Dr. J. H. J. Petrini and reproduced from Master, et al., 2001. Mol. Cell. Biol. 21: 6006-6016.
Application
Controls: normal rabbit IgG: (G), no antibody (H), input DNA (I) and mock (J). Data kindly provided by J. L. Lavrrar and reproduced from J. Biol. Chem. 279: 46343-46349, 2004, with permission from the American Society for Biochemistry and Molecular Biology.
Application
Antibodies tested include E2F-1 (C-20): (A), E2F-2 (C-20): (B), E2F-3 (C-18): , E2F-3 (N-20): (C) and E2F-4 (C-20): (D). Data kindly provided by Dr. J. H. J. Petrini and reproduced with permission from Master, et al. 2001. Mol. Cell. Biol. 21: 6006-6016.
Application
Western blot analysis of BALB/3T3 cells transfected with either y-CRE (A) or y-CRE/E2F (B).| Application Notes | recommended for detection of E2F-1 and, to a lesser extent, E2F-2 and E2F-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
