Application
Western blot analysis of CRALBP expression in non-transfected: (A) and mouse CRALBP transfected: (B) 293T whole cell lysates and rat eye tissue extract (C).Application
Western blot analysis of CRALBP expression in rat eye tissue extract.| Product Name | CRALBP Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-100 mapping at the N-terminus of CRALBP of human origin |
| Modification Notes | 11-cis-retinal, the universal chromophore of the vertebrate retina, is coupled to opsins in both rod and cone photoreceptor cells and is photoisomerized to all-trans-retinal by light. This conversion is inhibited when 11-cis-retinol is in a complex with cellular retinaldehyde-binding protein (CRALBP). CRALBP may play a role in the vertebrate visual process as a substrate-routing protein, influencing the enzymatic partitioning of 11-cis-retinol at a key branch point in the visual cycle. Human CRALBP maps to chromosome 15q26 and encodes a 316 amino acid protein. CRALBP is not expressed in photoreceptors and is abundant in the retinal pigment epithelium (RPE) and Muller cells of the neuroretina, where it carries 11-cis-retinol and 11-cis-retinaldehyde. Mutations in the human CRALBP gene cause retinal pathology and delayed dark adaptation. CRALBP knockout mice have a delayed response in rhodopsin regeneration, 11-cis-retinal production and dark adaptation after illumination. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CRALBP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-100 mapping at the N-terminus of CRALBP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-100 mapping at the N-terminus of CRALBP of human origin |
| Entrez Gene | 6017 |
|---|
Application
Western blot analysis of CRALBP expression in non-transfected: (A) and mouse CRALBP transfected: (B) 293T whole cell lysates and rat eye tissue extract (C).
Application
Western blot analysis of CRALBP expression in rat eye tissue extract.| Application Notes | recommended for detection of CRALBP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
