Application
Western blot analysis of Parkin expression in SH-SY5Y whole cell lysate.| Product Name | Parkin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 61-360 mapping within an internal region of Parkin of human origin |
| Modification Notes | Parkin is a zinc-finger protein that is related to ubiquitin at the amino terminus. The wild type Parkin gene, which maps to human chromosome 6q25.2-27, encodes a 465 amino acid full-length protein that is expressed as multiple isoforms. Mutations in the Parkin gene are responsible for autosomal recessive juvenile Parkinson’s disease and commonly involve deletions of exons 3-5. In humans, Parkin is expressed in a subset of cells of the basal ganglia, midbrain, cerebellum and cerebral cortex, and is subject to alternative splicing in different tissues. Parkin expression is also high in the brainstem of mice, with the majority of immunopositive cells being neurons. The Parkin gene has been identified in a diverse group of organisms including mammals, birds, frog and fruit flies, suggesting that analogous functional roles of the Parkin protein may have been highly conserved during the course of evolution. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Parkin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 61-360 mapping within an internal region of Parkin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 61-360 mapping within an internal region of Parkin of human origin |
| Entrez Gene | 5071 |
|---|
Application
Western blot analysis of Parkin expression in SH-SY5Y whole cell lysate.| Application Notes | recommended for detection of Parkin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
