Application
Western blot analysis of ISGF-3γ p48 expression in non-transfected: (A) and human ISGF-3γ p48 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ISGF-3γ p48 expression in non-transfected 293T: (A), mouse ISGF-3γ p48 transfected 293T: (B) and MCF7 (C) whole cell lysates.| Product Name | ISGF-3γ p48 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of ISGF-3γ p48 of human origin |
| Modification Notes | Interferon signaling to the cell nucleus operates through phosphorylation on Tyrosine of proteins that have been designated Stats (signal transducers and activators of transcription). The first members of this family to be described include Stat1å p91, Stat1∫ p84 (a form of p91 that lacks 38 COOH-terminal amino acids) and Stat2 p113. Other members of the family include Stat3, which becomes activated through phosphorylation on Tyrosine as a DNA binding protein in response to epidermal growth factor (EGF) and interleukin-6 (IL-6) but not interferon g (IFN-©) and Stat4. Stat1å p91 (or Stat1∫ p84) and p113 form a complex (designated ISGF-3) with p48, a protein that has been shown by sequence analysis to be a member of the interferon regulatory (IRF) family of DNA binding proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of ISGF-3γ p48 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of ISGF-3&gamma p48 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of ISGF-3γ p48 of human origin |
| Entrez Gene | 10379 |
|---|
Application
Western blot analysis of ISGF-3γ p48 expression in non-transfected: (A) and human ISGF-3γ p48 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ISGF-3γ p48 expression in non-transfected 293T: (A), mouse ISGF-3γ p48 transfected 293T: (B) and MCF7 (C) whole cell lysates.| Application Notes | recommended for detection of ISGF-3γ p48 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
